Composite

Part:BBa_K5226063:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-10)


Mmp1 Vib C1M-porin58 Vib C3M


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1199
    Illegal EcoRI site found at 3904
    Illegal EcoRI site found at 5277
    Illegal PstI site found at 4846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1199
    Illegal EcoRI site found at 3904
    Illegal EcoRI site found at 5277
    Illegal NheI site found at 2161
    Illegal NheI site found at 4200
    Illegal PstI site found at 4846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1199
    Illegal EcoRI site found at 3904
    Illegal EcoRI site found at 5277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1199
    Illegal EcoRI site found at 3904
    Illegal EcoRI site found at 5277
    Illegal PstI site found at 4846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1199
    Illegal EcoRI site found at 3904
    Illegal EcoRI site found at 5277
    Illegal PstI site found at 4846
    Illegal NgoMIV site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

PCR amplification.Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009977.1）

References